Ayalew Girmay, Meresa Lema, Alemu Tsegaye, Yesuf Ebrahim, Ertiban Desale
Abstract
Honey bees need pollen and nectar to survive, and strong colonies before colony buildup season require proper feeding to support brood development. This study evaluated the impacts of supplements with varying protein content on colony performance, brood rearing, and honey production. Sixteen colonies were divided into four groups: three treatments and one control, each with four replicates. Treatments included Acacia pod flour: pollen (9:1 w/w), pea flour: pollen (9:1 w/w), and a mix of Acacia pod and pea flour: pollen (9:1 w/w); the control group received no supplementation. Feed was given in feeder supper box hives and measured every 7 days, with intake and refusal recorded. Hive resources such as pollen, nectar, brood space (cm2), adult bees, and honey were assessed using a frame-sized grid meter at the Gazgibilla apiary research site. Feed types were selected through palatability testing. The mixture of Acacia pod and pea flour had the highest consumption (243.19 g/day), brood area, pollen area, nectar area, colony size (frames covered with bees), and honey yield (35 kg/colony). The control group had the lowest values on all measures. Supplementary feeding increased honeybee populations and improved colony performance during dearth periods. The author recommends the commercial use of the Acacia and pea diet mix for sustained colony growth during the dearth periods.
Full Text:
References
Abebe Jenberie, A. B. and K. K. (2014). Floral phenology and pollen potential of honey bee plants in North- East dry land areas of Amhara region , Ethiopia. 7(5), 36–49.
Abdo l r e za Sa ffar i 1 , Pe t e r G. Kevan 1, J. s L. A. ki ns on 2 1. (2010). PALATABILITY AND CONSUMPTION OF PATTY-FORMULATED POLLEN AND POLLEN SUBSTITUTES AND THEIR EFFECTS ON HONEYBEE COLONY PERFORMANCE. 54(2), 63–71.
Adanacioğlu, H., Pocol, C. B., & Bay, V. (2022). Economic Analysis of Honey Bee Colonies Fed with Different Pollen Diets before Wintering. 0225(2), 217–227. https://doi.org/10.18615/anadolu.1173055
Adgaba, N., Al-ghamdi, A., Sharma, D., Tadess, Y., Alghanem, S. M., Ali, K., Javed, M., Khalid, G., & Mohamed, A. (2020). Saudi Journal of Biological Sciences Physico-chemical , antioxidant and anti-microbial properties of some Ethiopian mono-floral honeys. Saudi Journal of Biological Sciences, 27(9), 2366–2372. https://doi.org/10.1016/j.sjbs.2020.05.031
Alemu Tsegaye, Abebe Jenberie, Lemma, M., & Addisu Bihonegn and Ayalew Girmay et al. (2018). Assessing the effect of adulteration on honey quality and major adulterants along the value chain in Eastern Amhara Region.
Al-ghamdi, A. (2016). Influence of different proteinaceous diets on consumption , brood rearing , and honey bee quality parameters under isolation conditions. 40(4). https://doi.org/10.3906/vet-1507-28
Ahamad, B., Kumari, I., Ahmad, Y., Sharma, B., Kumar, R., Fahad, M., Abul, M., & Masood, J. (2021). Saudi Journal of Biological Sciences Honeybee nutrition and pollen substitutes : A review. Saudi Journal of Biological Sciences, 28(1), 1167–1176. https://doi.org/10.1016/j.sjbs.2020.11.053
Ahmad, S. K., Ali, A., Anwar, P. T., & Dawah, H. A. (2023). Journal of Experimental Biology and Agricultural Sciences An overview of artificial nutrition in apiculture. 11(2320), 884–918.
Com, D., & Musumhi, T. (2013). Regional Lessons Learned for Somalia in the Apiculture ( Beekeeping ) Sector. March.
Dodologlu, A., Emsen, B., Dodologlu, A., & Emsen, B. (2011). Effect of Supplementary Feeding on Honey Bee Colony. 2119, 20–22. https://doi.org/10.1080/09712119.2007.9706878
Ebrahim, Y., Kibret, M., & Meaza, M. (2025). Evaluation of the Physicochemical Characteristics of Honey Produced by Honey Bees ( Apis mellifera ) in Waghimra Zone. 06(03), 10–15.
El-din, H. S., & Al-fattah, M. A. A. A. (2023). The type of protein supplement or substitute and its location from brood affect honey bee colony performance. 4(1), 125–134. https://doi.org/10.21608/sjas.2022.114530.1179
El-wahab, A. (2018). Impact of some Pollen Substitutes in Liquid Form on the Biological Activities of Honey Bee Colonies. 12(6), 1035–1041.
El-din, H. S., & Al-fattah, M. A. A. A. (2022). The type of protein supplement or substitute and its location from brood affect honey bee colony performance. 4(1), 125–134. https://doi.org/10.21608/sjas.2022.114530.1179
El-wahab, A. (2016). Assessment a New Pollen Supplement Diet for Honey Bee Colonies and Their Effects on some Biological Activities. 12(1), 55–62.
Fagúndez, G. A., Caccavari, M. A., & Fagu, G. A. (2007). Pollen analysis of honeys from the central zone of the Argentine province of Entre Ríos Pollen analysis of honeys from the central zone of the Argentine ´ os. 3134. https://doi.org/10.1080/00173130601086636 October.
Fikadu, A. A., Tilaye, K. A., Mebrat, Mu. A., & Elimnh, L. A. (2017). Adoption and Intensity of Use of Modern Beehives in Wag Himra and North Wollo Zzones, Amhara Region, Ethiopia. Ethiopian Journal of Economics, 26(2), 1–30.
Gasim, S. M., & Abdelmula, A. A. (2018). Impact of Bee Pollination on Yield of Faba Bean ( Vicia faba L .) Grown under Semi-Arid Conditions. 729–740. https://doi.org/10.4236/as.2018.96051
Gemechis Legesse, Kibebew Wakjira, Amssalu Bezabeh, Desalegn Begna, & Admassu Addi et al. (2012). OROMIA AGRICULTURAL RESEARCH INSTITUTE HOLETA BEE RESEARCH CENTER APICULTURE RESEARCH ACHIEVEMENTS IN ETHIOPIA.
Hunde, T. A. (2022). Evaluation of Pollen Supplementary Diets for Honeybee ( Apis mellifera ) Colonies and Their Effects on Some Biological Activities. 10(3), 54–60. https://doi.org/10.11648/j.abb.20221003.11
Jong, D. De, Junqueira, E., Kevan, P. G., & Atkinson, J. L. (2014). Pollen substitutes increase honey bee haemolymph protein levels as much as or more than does pollen Pollen substitutes increase honey bee haemolymph protein levels as much as or more than does pollen . February 2009. https://doi.org/10.3896/IBRA.1.48.1.08
Kendall, A., Yuan, J., Brodt, S., & Jan, K. (n.d.). Carbon Footprint of U . S . Honey Production and Packing Report to the National Honey Board. 1–23.
Lema, M., Tsegaye, A., Tsegaye, A. T., Bihonegn, A., Ibrahim1, Y., Desale, E., & Girmay, A. (2025). Identifying and Evaluating the Effect of Poisonous Plants to Honeybee Colonies in East Amhara, Ethiopia. International Journal on Food, Agriculture and Natural Resources, 6(1), 55–58. https://doi.org/10.46676/ij-fanres.v6i1.429
Livestock Research Directorate. (2019).
Lorato, Y. (2023). Review on the Status of Honey Bee Feeding Practice and Honey Production Potential : Ethiopia Review on the Status of Honey Bee Feeding Practice and Honey Production Potential : Ethiopia.
Masaka, K. (2023). Yearly Fluctuations in Honey Production in Hokkaido , Northern Japan , with Special Reference to Weather Conditions and Masting Behavior. 04(03), 62–68.
Melisie, D., Damte, T., & Thakur, A. K. (2016). Farmers ’ insecticide use practice and its effect on honeybees ( Apis mellifera ) foraging on onion flower in Adami Tullu district of Ethiopia. 4(1), 139–145.
Pereira, J., Mohallem, L., Esposito, E., De, G. I., Souza, M. H. De, Francoy, T. M., Morais, M. M., Pereira, J., Mohallem, L., Esposito, E., Inácio, G., Honorato, D. M., Pereira, J., Mohallem, L., & Sposito, E. E. (2020). Effects of ensiling on the quality of protein supplements for honey bees Apis mellifera To cite this version : HAL Id : hal-02883282 Original article Effects of ensiling on the quality of protein supplements for honey bees Apis mellifera. https://doi.org/10.1007/s13592-019-00661-4
Offman, G. D. E. G. R., Hen, Y. C., Ivera, R. R., Arroll, M. C., Hambers, M. C., Idalgo, G. H., & Ong, E. W. D. J. (2016). Original article Honey bee colonies provided with natural forage have lower pathogen loads and higher overwinter survival than those fed protein supplements. 186–196. https://doi.org/10.1007/s13592-015-0386-6
Radev, Z., Liolios, V., Tananaki, C., & Thrasyvoulou, A. (2014). The impact of the nutritive value of9pollen on the development , reproduction and productivity of honey bee ( Apis Mellifera L .) THE IMPACT OF THE NUTRITIVE VALUE OF POLLEN ON THE DEVELOPMENT , REPRODUCTION AND PRODUCTIVITY OF HONEY BEE ( APIS MELLIFERA . April 2023.
Tesfaye, O. (2019). The Effect of Pollen Supplementary Feeding on the Production of Honeybee ( Apis mellifera ) During Dearth Periods Under Haro Sabu Condition of Kellem Wollega Zone , Western Ethiopia. 9(9), 1–6. https://doi.org/10.7176/JBAH
Tesfaye, O., Mekonnen, E., & Dida, A. (2023). Botanical Origin and Physicochemical Composition of Apis mellifera L. Honey from Western Oromia, Ethiopia. Asian Journal of Biological Sciences, 16(1), 40–51. https://doi.org/10.3923/ajbs.2023.40.51
Topal, E., Rodica, M., & Bay, V. (2022). The Effect of Supplementary Feeding with Different Pollens in Autumn on Colony Development under Natural Environment and In Vitro Lifespan of Honey Bees.
Tolera Kumsa, & Dejene Takele. (2014). Assessment of the Effect of Seasonal Honeybee Management on Honey Production of Ethiopian Honeybee (Apis mellifera) in Modern Beekeeping in Jimma Zone. Journal of Agriculture and Environmental Management, 3(5), 246–254.
Umrao, R. K., Singh, R., Ahmad, M., & Singh, T. N. (2011). Stability Analysis of Cut Slopes Using Continuous Slope Mass Rating and Kinematic Analysis in Rudraprayag District, Uttarakhand. Geomaterials, 01(03), 79–87. https://doi.org/10.4236/gm.2011.13012.
Walle, Y., Shibabaw, W., & Tesema, Z. (2025). Phenotypic and Genetic Parameter Estimation of Milk Traits of Abergelle Goat in Ethiopia. 06(02), 30–36
Wubie, A. J., Bezabeh, A., & Kebede, K. (2014). Floral phenology and pollen potential of honey bee plants in North- East dry land areas of Amhara region , Ethiopia. 7(5), 36–49.
Zone, W., Alebachew, W., Id, A., Mersso, B. T., Sisay, T. A., Arega, A. B., & Alene, A. T. (2024). Effect of various supplements on productive performance of honey bees , in the south. 1–16. https://doi.org/10.1371/journal.pone.0303579
DOI: https://doi.org/10.46676/ij-fanres.v6i4.578
Download .PDF (Mirror 1) | Download .PDF (Mirror 2)
There are currently no refbacks.
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.