Ganesamoorthy Janarny, Somathilaka Ranaweera, Prasanna Priyantha Gunathilake
Abstract
Cassia fistula is a well-known plant species for the medicinal use of it leaves, barks and flowers. It has been identified as a rich source of phenolics which can be attributed to its medicinal properties. However, these phenolics should be extracted appropriately to effectively utilize them in various applications. This study focused on maximizing the extraction yield of total phenolics and total anthocyanins along with maximum 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity from edible flowers of C.fistula as a function of solid:liquid ratio, ethanol concentration, extraction temperature and time. The data was subjected to response surface methodology and the corresponding second order polynomial models were generated. The results showed that the polynomial models for all responses were significant, did not show lack of fit and presented determination of coefficients above 95%. This indicates the suitability of the models for prediction purposes. Using desirability function, the optimum extraction process parameters to obtain maximum values of all responses was found to be 29.56% ethanol, 1:45 solid to liquid ratio at extraction temperature of 31°C and time 24 minutes. Under these optimal conditions, the experimental values were in close agreement with the predicted values and did not show any significant difference (p<0.05). The identified optimized conditions could be used to extracts phenolics from C.fistula flowers in a cost effective and efficient manner.
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References
Mwangi, R. W., Macharia, J. M., Wagara, I. N., & Bence, R. L. (2021). The medicinal properties of Cassia fistula L: A review. Biomed. Pharmacother, 144, 112240.
Nawwal, K., Saeed, A., Hussain, T., Ajmal, S., Choudry, A., Umair, M., ... & Khan, M. A. (2021). Phytochemical evaluation and antioxidant activities in flower and leaves of Cassia fistula and Terminalia arjuna. PJBB, 2(2), 195-206.
Neelam, C., Ranjan, B., Komal, S., Nootan, C., 2011. Review on Cassia fistula. Int. J. Res. Ayurveda Pharm. 2, 426-430.
Danish, M., Singh, P., Mishra, G., Srivastava, S., Jha, K., Khosa, R., 2011. Cassia fistula Linn. (Amulthus)—an important medicinal plant: a review of its traditional uses, phytochemistry and pharmacological properties. J. Nat.
Prod. Plant Resour. 1(1), 101-118.
Sharma, A., Kumar, A., & Jaitak, V. (2021). Pharmacological and chemical potential of Cassia fistula L-a critical review. J.Herb.Med. 26, 100407.
P. Chandra, R. Pandey, B. Kumar, M. Srivastva, P. Pandey, J. Sarkar, B.P. Singh, Quantification of multianalyte by UPLC-QqQLIT-MS/MS and in-vitro anti-proliferative screening in Cassia species, Ind. Crop. Prod. 76 (2015) 1133–1141. doi.org/10.1016/j.indcrop.2015.08.030.
T. Bahorun, V.S. Neergheen, O.I. Aruoma, Phytochemical constituents of Cassia fistula, Afr. J. Biotechnol. 4 (13) (2005) 1530–1540, https://doi.org/10.4314/ajfand.v4i13.71772.
G.C.G. Martínez- ́avila, C. Castro-l ́opez, R. Rojas, G.C.G. Martínez, Science screening of the Cassia fistula phytochemical constituents, Ann. Nutr. Food 2 (2) (2018) 1–3
Ongkowijoyo, P., Luna-Vital, D. A., de Mejia, E. G. (2018). Extraction techniques and analysis of anthocyanins from food sources by mass spectrometry: An update. Food chemi. 250, 113-126.
Alharbi, N. K., Al-Zaban, M. I., Albarakaty, F. M., Abdelwahab, S. F., Hassan, S. H., & Fawzy, M. A. (2022). Kinetic, Isotherm and Thermodynamic Aspects of Zn2+ Biosorption by Spirulina platensis: Optimization of Process Variables by Response Surface Methodology. Life, 12(4), 585.
Janarny, G., Ranaweera, K. K. D. S., & Gunathilake, K. D. P. P. (2022). Digestive recovery of polyphenols, antioxidant activity, and anti‐inflammatory activity of selected edible flowers from the family Fabaceae. J.Food Biochem. e14052.
Janarny, G., Ranaweera, K. K. D. S., & Gunathilake, K. D. P. P. (2021). Antioxidant activities of hydro-methanolic extracts of Sri Lankan edible flowers. Biocatal. Agric. Biotechnol, 35, 102081
Ӧztürk, H., Kolak, U., Meric, C., (2011). Antioxidant, anticholinesterase and antibacterial activities of Jurinea consanguinea DC. Rec. Nat. Prod. 5 (1), 43.
Ferreira, S. L. C., Bruns, R. E., da Silva, E. G. P., Dos Santos, W. N. L.,
Quintella, C. M., David, J. M., ... & Neto, B. B. (2007). Statistical designs and response surface techniques for the optimization of chromatographic systems. J.Chromatogr. A, 1158(1-2), 2-14.
Yolmeh, M., & Jafari, S. M. (2017). Applications of response surface methodology in the food industry processes. Food and Bioproc Tech, 10(3), 413-433.
Cacace, J. E., & Mazza, G. (2003). Optimization of extraction of anthocyanins from black currants with aqueous ethanol. J.Food Sci. 68(1), 240-248.
Chen X, Wu X, Chai W, et al. (2013) Optimization of extraction of phenolics from leaves of Ficus virens. Journal of Zheijang University Biomedicine & Biotechnology 14:903–915. doi:10.1631/jzus. B1200365
Gunathilake, K. D. P. P., Ranaweera, K. K. D. S., Rupasinghe, H. P. V. (2019). Response surface optimization for recovery of polyphenols and carotenoids from leaves of Centella asiatica using an ethanol‐based solvent system. Food Sci. Nutr, 7(2), 528-536.
Alara, O.R., Abdul Mudalip, S.K., Olalere, O.A., 2017. Optimization of mangiferin extrated from Phaleria macrocarpa fruits using response surface methodology. J.Appl. Res. Med. and Aromat Plants. 5, 82-87.
Fu, Q., Zhang, L., Cheng, N., Jia, M., & Zhang, Y. (2014). Extraction optimization of oleanolic and ursolic acids from pomegranate (Punica granatum L.) flowers. Food and Bioprod. Process, 92(3), 321-327.
Fan, G., Han, Y., Gu, Z., & Chen, D. (2008). Optimizing conditions for anthocyanins extraction from purple sweet potato using response surface methodology (RSM). LWT-Food Sci. and Technol. 41(1), 155-160.
Thoo, Y. Y., Ho, S. K., Liang, J. Y., Ho, C. W., Tan, C. P. (2010). Effects of binary solvent extraction system, extraction time and extraction temperature on phenolic antioxidants and antioxidant capacity from mengkudu (Morinda citrifolia). Food Chem., 120(1), 290-295.
Deng, J., Liu, Q., Zhang, C., Cao, W., Fan, D., & Yang, H. (2016). Extraction optimization of polyphenols from waste kiwi fruit seeds (Actinidia chinensis Planch.) and evaluation of its antioxidant and anti-inflammatory properties. Molecules, 21(7), 832.
Patil, G., Madhusudhan, M. C., Babu, B. R., Raghavarao, K. S. M. S. (2009). Extraction, dealcoholization and concentration of anthocyanin from red radish. Chem. Eng. Process. Process Intensification, 48(1), 364-369.
Sulaiman, I. S. C., Basri, M., Masoumi, H. R. F., Chee, W. J., Ashari, S. E., Ismail, M. (2017). Effects of temperature, time, and solvent ratio on the extraction of phenolic compounds and the anti-radical activity of Clinacanthus nutans Lindau leaves by response surface methodology. Chem. Cent. J, 11(1), 1-11
Kamarudin, A. A., Esa, N. M., Saad, N., Sayuti, N. H., Razak, N. A. A. (2020). Heat assisted extraction of phenolic compounds from Eleutherine bulbosa (Mill.) bulb and its bioactive profiles using response surface methodology. Ind. Crops Prod. 144, 112064.
Belwal, T., Dhyani, P., Bhatt, I. D., Rawal, R. S., Pande, V. (2016). Optimization extraction conditions for improving phenolic content and antioxidant activity in Berberis asiatica fruits using response surface methodology (RSM). Food Chem., 207, 115-124.
Pham, T. N., Lam, T. D., Nguyen, M. T., Le, X. T., Vo, D. V. N., Toan, T. Q., Vo, T. S. (2019, July). Effect of various factors on extraction efficiency of total anthocyanins from Butterfly pea (Clitoria ternatea L. Flowers) in Southern Vietnam. In IOP Conference Series: Materials Science and Engineering (Vol. 544, No. 1, p. 012013). IOP Publishing.
He, B., Zhang, L. L., Yue, X. Y., Liang, J., Jiang, J., Gao, X. L., & Yue, P. X. (2016). Optimization of ultrasound-assisted extraction of phenolic compounds and anthocyanins from blueberry (Vaccinium ashei) wine pomace. Food Chem. 204, 70-76.
Chen, F., Sun, Y., Zhao, G., Liao, X., Hu, X., Wu, J. And Wang, Z. 2007. Optimization of ultrasound-assisted extraction of anthocyanins in red raspberries and identification of anthocyanins in extract using high-performance liquid chromatography–mass spectrometry. Ultrason. Sonochem. 14, 767–778
DOI: https://doi.org/10.46676/ij-fanres.v4i1.94
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